404D
CRYSTAL STRUCTURE OF THE RNA/DNA HYBRID R(GAAGAGAAGC). D(GCTTCTCTTC)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Detector | PRINCETON 2K |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.720, 45.550, 47.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.181 |
| Rwork | 0.184 |
| RMSD bond length | 0.014 * |
| RMSD bond angle | 0.047 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.056 |
| Number of reflections | 1881 |
| Completeness [%] | 86.6 |
| Redundancy | 4.06 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 293 | pH 7.00, VAPOR DIFFUSION, temperature 293.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | HEPES | ||
| 2 | 1 | 1 | PEG4000 | ||
| 3 | 1 | 1 | AMMONIUM ACETATE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 2 | HEPES | ||
| 6 | 1 | 2 | PEG4000 | ||
| 7 | 1 | 2 | AMMONIUM ACETATE | ||
| 8 | 1 | 2 | MGCL2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 50 (mM) | ||
| 2 | 1 | reservoir | 5 (%) | ||
| 3 | 1 | reservoir | ammonium acetate | 200 (mM) | |
| 4 | 1 | reservoir | magnesium acetate | 150 (mM) |
