3ZZW
Crystal structure of the kinase domain of ROR2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-19 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.613, 61.301, 79.936 |
| Unit cell angles | 90.00, 93.07, 90.00 |
Refinement procedure
| Resolution | 46.770 - 2.900 |
| R-factor | 0.1903 |
| Rwork | 0.187 |
| R-free | 0.24730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jiv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 3.060 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.170 | 0.830 |
| Number of reflections | 12094 | |
| <I/σ(I)> | 4.4 | 0.9 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5.7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2M AMMONIUM ACETATE, 2.2M AMMONIUM SULFATE, pH 7.5 |
