3ZZV
BambL complexed with Htype2 tetrasaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-22 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.740, 47.770, 74.600 |
| Unit cell angles | 90.00, 100.81, 90.00 |
Refinement procedure
| Resolution | 73.280 - 1.680 |
| R-factor | 0.21453 |
| Rwork | 0.211 |
| R-free | 0.27052 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wz0 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.861 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.280 | 1.770 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.040 | 0.350 |
| Number of reflections | 26045 | |
| <I/σ(I)> | 11.3 | 1.9 |
| Completeness [%] | 96.9 | 81.2 |
| Redundancy | 3.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.5 |
