3ZZU
Crystal structure of Staphylococcus aureus elongation factor G with mutations M16I and F88L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-06 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.200, 125.520, 106.900 |
| Unit cell angles | 90.00, 108.21, 90.00 |
Refinement procedure
| Resolution | 47.147 - 2.980 |
| R-factor | 0.2397 |
| Rwork | 0.237 |
| R-free | 0.29420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fnm |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.937 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.200 | 3.000 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Rmerge | 0.120 | 0.920 |
| Number of reflections | 31218 | |
| <I/σ(I)> | 8.13 | 1.8 |
| Completeness [%] | 93.2 | 76.4 |
| Redundancy | 3.7 | 3.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
