3ZZT
Crystal structure of Staphylococcus aureus elongation factor G with a fusidic-acid-resistant mutation F88L
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-09-06 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.970, 127.930, 107.440 |
Unit cell angles | 90.00, 107.86, 90.00 |
Refinement procedure
Resolution | 47.402 - 2.950 |
R-factor | 0.2434 |
Rwork | 0.241 |
R-free | 0.29370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fnm |
RMSD bond length | 0.004 |
RMSD bond angle | 0.903 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.400 | 3.000 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.080 | 0.950 |
Number of reflections | 32339 | |
<I/σ(I)> | 14.57 | 1.64 |
Completeness [%] | 90.2 | 72.4 |
Redundancy | 3.76 | 3.79 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |