3ZZ0
Crystal structure of ribosomal elongation factor (EF)-G from Staphylococcus aureus with a fusidic acid hyper-sensitivity mutation M16I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-09-06 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.940, 125.460, 106.270 |
Unit cell angles | 90.00, 107.52, 90.00 |
Refinement procedure
Resolution | 46.710 - 2.800 |
R-factor | 0.25 |
Rwork | 0.249 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fnm |
RMSD bond length | 0.004 |
RMSD bond angle | 0.965 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.700 | 3.000 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.140 | 0.860 |
Number of reflections | 36516 | |
<I/σ(I)> | 7.55 | 1.85 |
Completeness [%] | 91.1 | 76.5 |
Redundancy | 3.73 | 3.78 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |