3ZYW
Crystal structure of the first glutaredoxin domain of human glutaredoxin 3 (GLRX3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-04 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 34.524, 87.052, 90.705 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.950 - 1.840 |
| R-factor | 0.20241 |
| Rwork | 0.200 |
| R-free | 0.24974 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yan |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.382 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.620 | 1.940 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.130 | 0.700 |
| Number of reflections | 23870 | |
| <I/σ(I)> | 11.4 | 2.6 |
| Completeness [%] | 97.8 | 88.3 |
| Redundancy | 6.3 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M BIS-TRIS PH 5.5, 25% PEG3350 |
