3ZYR
Structure of the lectin from Platypodium elegans in complex with heptasaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-16 |
| Detector | MARRESEARCH SX-165 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.400, 76.980, 125.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.570 - 1.650 |
| R-factor | 0.15724 |
| Rwork | 0.156 |
| R-free | 0.18816 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IN30 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.595 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.560 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.050 | 0.250 |
| Number of reflections | 55022 | |
| <I/σ(I)> | 13.6 | 3.9 |
| Completeness [%] | 91.4 | 65.8 |
| Redundancy | 3.4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 10% PEG20K, 2% DIOXANE, 100 MM BICINE PH 9, 30% GLYCEROL ADDED AS CRYOPROTECTANT. |
