3ZYJ
NetrinG1 in complex with NGL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 184.420, 204.430, 288.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.440 - 3.250 |
| R-factor | 0.2567 |
| Rwork | 0.256 |
| R-free | 0.26830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zyg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.930 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 3.400 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.310 | 1.170 |
| Number of reflections | 39128 | |
| <I/σ(I)> | 8.8 | 1.5 |
| Completeness [%] | 91.3 | 73.3 |
| Redundancy | 5.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2% PEG 400, 2 M AMMONIUM SULPHATE, 0.1 M HEPES PH 7.5, ADDITIVE PEG 400. |
