3ZYI
NetrinG2 in complex with NGL2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND |
Synchrotron site | Diamond |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 75.420, 153.390, 158.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.950 - 2.600 |
R-factor | 0.2456 |
Rwork | 0.243 |
R-free | 0.28680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zyg |
RMSD bond length | 0.008 |
RMSD bond angle | 1.000 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.9.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.160 | 1.180 |
Number of reflections | 28390 | |
<I/σ(I)> | 16.6 | 2.1 |
Completeness [%] | 98.7 | 98.7 |
Redundancy | 9.2 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 50% V/V 2-METHYL-2, 4-PENTANEDIOL, 0.2 M AMMONIUM DI-HYDROGEN PHOSPHATE, 0.1 M TRIS PH 8.5. |