3ZYC

DYNAMIN 1 GTPASE GED FUSION DIMER COMPLEXED WITH GMPPCP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	95
Detector technology	CCD
Collection date	2010-09-15
Detector	RIGAKU-MSC SATURN 200
Spacegroup name	P 1 21 1
Unit cell lengths	44.151, 82.635, 95.014
Unit cell angles	90.00, 96.68, 90.00

Refinement procedure

Resolution	30.000 - 2.200
R-factor	0.2253
Rwork	0.225
R-free	0.26910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x2e
RMSD bond length	0.006
RMSD bond angle	2.140
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.260
High resolution limit [Å]	2.200	2.200
Rmerge	0.080	0.340
Number of reflections	34206
<I/σ(I)>	12.23	2.95
Completeness [%]	99.0	90.5
Redundancy	4.27	2.57

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	GGGMPPCP WAS CRYSTALLIZED BY SITTING DROP VAPOR DIFFUSION IN 0.1M HEPES PH 7.0, 200 MM MGCL2, AND 24-30% PEG 2000 MME USING A DROP SIZE OF 3 MICROL AND RESERVOIR VOLUME OF 65 MICROL. CRYSTALS GREW IN 3-5 DAYS AT 20C. THE ADDITION OF 200 MM LICL, NACL, KCL, OR NH4CL TO THE RESERVOIR SOLUTION DRAMATICALLY IMPROVED THE CRYSTALS. CRYSTALS GROWN IN THE PRESENCE OF KCL SHOWED THE BEST DIFFRACTION.