3ZY6
Crystal structure of POFUT1 in complex with GDP-fucose (crystal-form-II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER |
Synchrotron site | ESRF |
Beamline | BM16 |
Temperature [K] | 90 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 144.583, 38.156, 68.101 |
Unit cell angles | 90.00, 102.85, 90.00 |
Refinement procedure
Resolution | 70.480 - 1.910 |
R-factor | 0.20314 |
Rwork | 0.202 |
R-free | 0.23733 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zy2 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.375 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.010 |
High resolution limit [Å] | 1.910 | 1.910 |
Rmerge | 0.100 | 0.520 |
Number of reflections | 39386 | |
<I/σ(I)> | 9.27 | 1.97 |
Completeness [%] | 98.9 | 96.5 |
Redundancy | 4.37 | 3.91 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |