3ZY1

Crystal structure of the human p63 tetramerization domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 4 2 2
Unit cell lengths	58.198, 58.198, 39.328
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.200 - 2.150
R-factor	0.23793
Rwork	0.236
R-free	0.28517
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.012
RMSD bond angle	1.354
Refinement software	REFMAC (5.6.0116)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.200	2.270
High resolution limit [Å]	2.150	2.150
Rmerge	0.050	0.800
Number of reflections	3966
<I/σ(I)>	23.7	3.3
Completeness [%]	99.4	99.8
Redundancy	9.4	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	SITTING DROP VAPOR DIFFUSION AT 17 DEGREE C. PROTEIN SOLUTION: 12-15 MG/ML IN 20 MM TRIS PH 8.5, 50 MM NACL CRYSTALLIZATION BUFFER: 10% PEG 8000, 0.1 M HEPES PH 7.5, AND 0.2 M CA ACETATE