3ZXY
Structure of S218A mutant of the protease domain of PatA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | I 2 3 |
Unit cell lengths | 130.610, 130.610, 130.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 92.360 - 1.580 |
R-factor | 0.15643 |
Rwork | 0.156 |
R-free | 0.17432 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dbi |
RMSD bond length | 0.008 |
RMSD bond angle | 1.387 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 92.360 | 1.620 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.080 | 0.650 |
Number of reflections | 48227 | |
<I/σ(I)> | 14.2 | 3.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.3 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |