3ZX6
Structure of Hamp(AF1503)-Tsr fusion - Hamp (A291V) mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH MX-225 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.220, 80.020, 139.170 |
| Unit cell angles | 90.00, 110.78, 90.00 |
Refinement procedure
| Resolution | 29.480 - 2.650 |
| R-factor | 0.2314 |
| Rwork | 0.229 |
| R-free | 0.28263 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zrx |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.940 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.750 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.100 | 0.600 |
| Number of reflections | 32412 | |
| <I/σ(I)> | 9.57 | 2 |
| Completeness [%] | 96.1 | 96.2 |
| Redundancy | 2.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | PH 7 |
