3ZW5
Crystal structure of the human Glyoxalase domain-containing protein 5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-04 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.490, 66.040, 76.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.090 - 1.600 |
R-factor | 0.1732 |
Rwork | 0.172 |
R-free | 0.19940 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.050 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.490 | 1.690 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.090 | 0.410 |
Number of reflections | 35137 | |
<I/σ(I)> | 10.6 | 3.8 |
Completeness [%] | 99.5 | 98.2 |
Redundancy | 5.5 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 2M AMMONIUM SULFATE, 2.5W/V PEG 400, PH7 HEPES |