3ZW0
Structure of BambL lectin from Burkholderia ambifaria
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-16 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.110, 47.250, 98.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.160 - 1.600 |
| R-factor | 0.14569 |
| Rwork | 0.143 |
| R-free | 0.18842 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bt9 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.537 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.240 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.030 | 0.060 |
| Number of reflections | 26647 | |
| <I/σ(I)> | 26.6 | 13.4 |
| Completeness [%] | 93.7 | 70.5 |
| Redundancy | 3.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.5 |
