3ZVX
STRUCTURE OF THE LECTIN FROM PLATYPODIUM ELEGANS IN COMPLEX WITH A TRIMANNOSIDE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-10 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.620, 80.680, 148.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.250 - 2.100 |
R-factor | 0.19785 |
Rwork | 0.195 |
R-free | 0.25180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1n3o |
RMSD bond length | 0.015 |
RMSD bond angle | 1.544 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.600 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.080 | 0.330 |
Number of reflections | 28894 | |
<I/σ(I)> | 9.3 | 2 |
Completeness [%] | 92.7 | 70.1 |
Redundancy | 3.4 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 1.5 M AMMONIUM SULFATE, 100 MM TRIS PH 8.5, 12% GLYCEROL. GLYCEROL CONCENTRATION WAS INCREASED TO 25% FOR CRYOPROTECTION |