3ZVS
Crystal structure of Archaemetzincin (AmzA) from Archaeoglobus fulgidus at 1.4 A resolution complexed with malonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.302, 63.490, 64.533 |
| Unit cell angles | 71.39, 86.68, 82.67 |
Refinement procedure
| Resolution | 31.542 - 1.396 |
| R-factor | 0.1441 |
| Rwork | 0.144 |
| R-free | 0.16360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2XHQ |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.434 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.540 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.030 | 0.240 |
| Number of reflections | 96247 | |
| <I/σ(I)> | 13.6 | 3.08 |
| Completeness [%] | 91.8 | 85.8 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 18 % PEG 3350 0.2 M SODIUM MALONATE PH 6.0 |
