3ZUZ
Structure of Shq1p C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.374, 50.092, 52.083 |
| Unit cell angles | 110.46, 98.85, 97.32 |
Refinement procedure
| Resolution | 21.960 - 1.500 |
| R-factor | 0.1724 |
| Rwork | 0.171 |
| R-free | 0.19400 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.960 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXE |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | 0.430 |
| Number of reflections | 54338 | |
| <I/σ(I)> | 14.5 | 2.6 |
| Completeness [%] | 93.9 | 92.4 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | CRYSTALLIZED AT 291 KELVIN BY THE SITTING DROP VAPOR DIFFUSION METHOD FROM A 1:1 MIXTURE OF PROTEIN AND PRECIPITANT CONTAINING 20 TO 25 % PEG 4000, 10 % ISOPROPANOL, 100 MM HEPES PH 7.5, 50 MM MGSO4 |
