3ZUX
Crystal structure of a bacterial homologue of the bile acid sodium symporter ASBT.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-01 |
Detector | ADSC CCD |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 73.330, 73.330, 163.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.640 - 2.200 |
R-factor | 0.1988 |
Rwork | 0.197 |
R-free | 0.22860 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.010 |
RMSD bond angle | 1.050 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.000 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.080 | 0.860 |
Number of reflections | 23427 | |
<I/σ(I)> | 8.8 | 1.2 |
Completeness [%] | 99.7 | 99.2 |
Redundancy | 4.32 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.5 | 50 MM SODIUM CITRATE PH 4.5, 70 MM NACL, 22-24% PEG400. |