3ZUU
The structure of OST1 (D160A, S175D) kinase in complex with gold
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 77.570, 99.017, 108.451 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.225 - 2.700 |
| R-factor | 0.2399 |
| Rwork | 0.238 |
| R-free | 0.27900 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.153 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.570 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.080 | 0.800 |
| Number of reflections | 23628 | |
| <I/σ(I)> | 22.6 | 4.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.2 | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M HEPES PH 7.0, 14% PEG 10000, AND 12% ETHYLENE GLYCOL. |
