3ZUG
E268D mutant of FAD synthetase from Corynebacterium ammoniagenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-06 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 3 |
Unit cell lengths | 133.545, 133.545, 133.545 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.050 |
R-factor | 0.19701 |
Rwork | 0.195 |
R-free | 0.22925 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x0k |
RMSD bond length | 0.009 |
RMSD bond angle | 1.083 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.190 | 2.160 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.060 | 0.510 |
Number of reflections | 49927 | |
<I/σ(I)> | 34.4 | 7.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 21.7 | 21.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 1.5 M LI2SO4, 0.1 M HEPES PH 7.5 |