3ZTA
The bacterial stressosome: a modular system that has been adapted to control secondary messenger signaling
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 56.410, 56.410, 148.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.725 - 2.700 |
| R-factor | 0.2167 |
| Rwork | 0.214 |
| R-free | 0.27510 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.734 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.720 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.070 | 0.370 |
| Number of reflections | 12435 | |
| <I/σ(I)> | 49.2 | 16.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 52.3 | 55.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 1.0 M AMMONIUM SULPHATE, 0.1 M BIS-TRIS PROPANE, PH 5.5, 1% PEG 3350. |
