3ZT7
GlgE isoform 1 from Streptomyces coelicolor with beta-cyclodextrin and maltose bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-29 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 113.270, 113.380, 315.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.800 - 2.500 |
R-factor | 0.19305 |
Rwork | 0.192 |
R-free | 0.22117 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zst |
RMSD bond length | 0.014 |
RMSD bond angle | 1.447 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0101) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.420 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.140 | 0.690 |
Number of reflections | 140547 | |
<I/σ(I)> | 10 | 2.3 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 4.9 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | pH 7.0 |