3ZT5
GlgE isoform 1 from Streptomyces coelicolor with maltose bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 113.820, 113.570, 315.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.010 - 2.090 |
| R-factor | 0.20487 |
| Rwork | 0.204 |
| R-free | 0.22757 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zst |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.440 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0101) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.890 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.120 | 0.430 |
| Number of reflections | 236917 | |
| <I/σ(I)> | 12 | 3.2 |
| Completeness [%] | 99.5 | 96.7 |
| Redundancy | 7.3 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7.0 |
