3ZSU
Structure of the CyanoQ protein from Thermosynechococcus elongatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-27 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 47.165, 47.165, 106.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.850 - 1.600 |
| R-factor | 0.164 |
| Rwork | 0.162 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ls0 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.937 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.350 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.090 | 0.620 |
| Number of reflections | 19307 | |
| <I/σ(I)> | 10.85 | 3.72 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 6.98 | 7.13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.8 M AMMONIUM SULFATE |
