3ZST
GlgE isoform 1 from Streptomyces coelicolor with alpha-cyclodextrin bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-29 |
Detector | ADSC CCD |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 113.240, 113.240, 314.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 106.540 - 2.300 |
R-factor | 0.17401 |
Rwork | 0.173 |
R-free | 0.20132 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | SEMET DERIVATIVE MODEL SOLVED BY MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.529 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | REFMAC (5.6.0101) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.350 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.090 | 0.330 |
Number of reflections | 85772 | |
<I/σ(I)> | 23.6 | 7.9 |
Completeness [%] | 93.7 | 66 |
Redundancy | 14.3 | 13.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | pH 7.0 |