3ZSS

Apo form of GlgE isoform 1 from Streptomyces coelicolor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-02
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	113.090, 112.980, 314.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	314.440 - 1.800
R-factor	0.23224
Rwork	0.231
R-free	0.24885
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zst
RMSD bond length	0.013
RMSD bond angle	1.339
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.6.0101)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.150	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.110	0.760
Number of reflections	351739
<I/σ(I)>	13.4	2.1
Completeness [%]	95.0	72.9
Redundancy	7.6	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		pH 7.0