3ZSS
Apo form of GlgE isoform 1 from Streptomyces coelicolor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-02 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 113.090, 112.980, 314.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 314.440 - 1.800 |
| R-factor | 0.23224 |
| Rwork | 0.231 |
| R-free | 0.24885 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zst |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.339 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0101) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.150 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.110 | 0.760 |
| Number of reflections | 351739 | |
| <I/σ(I)> | 13.4 | 2.1 |
| Completeness [%] | 95.0 | 72.9 |
| Redundancy | 7.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7.0 |
