3ZSL
Crystal structure of Apo Human Galectin-3 CRD at 1.08 angstrom resolution, at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-5 |
Synchrotron site | MAX II |
Beamline | I911-5 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-20 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.710, 58.250, 62.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.080 |
R-factor | 0.1501 |
R-free | 0.18240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zsj |
RMSD bond length | 0.013 |
RMSD bond angle | 0.032 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.110 |
High resolution limit [Å] | 1.080 | 1.080 |
Rmerge | 0.070 | 0.680 |
Number of reflections | 56940 | |
<I/σ(I)> | 11.6 | 2.4 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 5.6 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 30% PEG 4000, 0.1M MGCL2, 0.008M BETA MERCAPTOETHANOL, 0.1M TRIS-HCL, PH 7.5, 0.4M NASCN |