3ZSK
Crystal structure of Human Galectin-3 CRD with glycerol bound at 0.90 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-5 |
| Synchrotron site | MAX II |
| Beamline | I911-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-13 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.830, 58.200, 62.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 0.900 |
| R-factor | 0.1326 |
| R-free | 0.15010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zsj |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.032 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 0.920 |
| High resolution limit [Å] | 0.900 | 0.900 |
| Rmerge | 0.040 | 0.790 |
| Number of reflections | 93342 | |
| <I/σ(I)> | 20.4 | 2.1 |
| Completeness [%] | 95.8 | 87.3 |
| Redundancy | 5.89 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 30% PEG 4000, 0.1M MGCL2, 0.008M BETA MERCAPTOETHANOL, 0.1M TRIS-HCL, PH 7.5, 0.4M NASCN |
