3ZS9

S. cerevisiae Get3-ADP-AlF4- complex with a cytosolic Get2 fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-28
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	52.558, 77.317, 165.831
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.537 - 2.095
R-factor	0.185
Rwork	0.182
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2woj
RMSD bond length	0.007
RMSD bond angle	1.024
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.100	0.660
Number of reflections	39345
<I/σ(I)>	16.5	1.6
Completeness [%]	97.1	89.1
Redundancy	5.2	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		30% PEG 3350, 0.3 AMMONIUM ACETATE, 0.1 M BIS-TRIS, PH 6.0