3ZRX
The high resolution structure of a dimeric Hamp-Dhp fusion displays strong asymmetry
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.110, 57.400, 51.620 |
| Unit cell angles | 90.00, 107.32, 90.00 |
Refinement procedure
| Resolution | 18.840 - 1.250 |
| R-factor | 0.15309 |
| Rwork | 0.151 |
| R-free | 0.19987 |
| Structure solution method | SAD |
| Starting model (for MR) | 2asm |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.161 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.280 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.040 | 0.710 |
| Number of reflections | 52510 | |
| <I/σ(I)> | 18.3 | 1.8 |
| Completeness [%] | 90.7 | 90.1 |
| Redundancy | 4.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.02 M CALCIUM CHLORIDE 0.1 M SODIUM ACETATE 30% MPD, PH4.6 |
