3ZRV
The high resolution structure of a dimeric Hamp-Dhp fusion displays asymmetry - A291F mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.260, 56.790, 83.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.740 - 1.650 |
| R-factor | 0.199 |
| Rwork | 0.196 |
| R-free | 0.24000 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.766 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.060 | 0.590 |
| Number of reflections | 23404 | |
| <I/σ(I)> | 17.31 | 2.9 |
| Completeness [%] | 99.1 | 91.4 |
| Redundancy | 6.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M TRISODIUM CITRATE PH5.6, 20% PEG4000, 20% ISOPROPANOL, pH 7 |
