3ZRI
N-domain of ClpV from Vibrio cholerae
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-05 |
Detector | ADSC CCD |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 71.898, 72.990, 73.033 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.950 - 1.800 |
R-factor | 0.193 |
Rwork | 0.191 |
R-free | 0.21800 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.024 |
RMSD bond angle | 1.879 |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.050 | |
Number of reflections | 18149 | |
<I/σ(I)> | 24 | 4.3 |
Completeness [%] | 99.5 | 95.4 |
Redundancy | 7 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 291 | 0.2M NAACETATE PH 7.0, 20% (W/V) PEG 3350, 18C |