3ZRG
Crystal structure of RxLR effector PexRD2 from Phytophthora infestans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-29 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.450, 52.920, 53.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.700 - 1.750 |
R-factor | 0.19204 |
Rwork | 0.189 |
R-free | 0.24550 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.015 |
RMSD bond angle | 1.447 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.700 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.080 | 0.420 |
Number of reflections | 12601 | |
<I/σ(I)> | 26.3 | 9.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 15.8 | 16.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 2.6 M AMMONIUM SULPHATE, 0.1 M TRIS.HCL PH 7.5 AND 0.2 M AMMONIUM BROMIDE |