3ZQS
Human FANCL central domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-11 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.600, 102.520, 65.760 |
| Unit cell angles | 90.00, 94.09, 90.00 |
Refinement procedure
| Resolution | 44.265 - 2.000 |
| R-factor | 0.1845 |
| Rwork | 0.183 |
| R-free | 0.20660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k1l DRWD DOMAIN |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.103 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.210 |
| Number of reflections | 103454 | |
| <I/σ(I)> | 8.91 | 2.03 |
| Completeness [%] | 79.0 | 75 |
| Redundancy | 1.5 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.2M L-PROLINE, 0.1M SODIUM TRI CITRATE PH5.5, 2% PEG 3,350 |
