3ZQN
Crystal structure of the small terminase oligomerization core domain from a SPP1-like bacteriophage (crystal form 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 127.649, 127.649, 106.869 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 90.260 - 2.200 |
| R-factor | 0.19694 |
| Rwork | 0.196 |
| R-free | 0.22166 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zqm |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.093 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 2.270 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.110 | 0.690 |
| Number of reflections | 45661 | |
| <I/σ(I)> | 23.5 | 4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.8 | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M KCL, 0.05 M HEPES PH 7.5, 35 % PENTAERYTHITOL PROPOXYLATE (5/4 PO/OH) |
