3ZQI

Structure of Tetracycline repressor in complex with inducer peptide- TIP2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH MX-225
Spacegroup name	P 21 21 21
Unit cell lengths	56.700, 78.560, 103.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.880 - 1.500
R-factor	0.16176
Rwork	0.160
R-free	0.19479
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ns8
RMSD bond length	0.029
RMSD bond angle	2.481
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.590
High resolution limit [Å]	1.500	1.500
Rmerge	0.050	0.300
Number of reflections	71948
<I/σ(I)>	25.07	6.74
Completeness [%]	95.8	93.1
Redundancy	7.28	7.18

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		0.2 M MAGNESIUM ACETATE, 0.1 M SODIUM CACODYLATE, PH 7.0, 20% PEG 8000.