3ZQH
Structure of Tetracycline repressor in complex with inducer peptide- TIP3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH MX-225 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.350, 57.340, 57.810 |
| Unit cell angles | 90.00, 112.29, 90.00 |
Refinement procedure
| Resolution | 24.720 - 1.600 |
| R-factor | 0.18273 |
| Rwork | 0.180 |
| R-free | 0.22401 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ns8 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.222 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.050 | 0.540 |
| Number of reflections | 28325 | |
| <I/σ(I)> | 12.8 | 1.71 |
| Completeness [%] | 96.1 | 80.1 |
| Redundancy | 3.47 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 0.2 M NACL, 0.1 M TRIS PH 9.0, 25% PEG 3350. |
