3ZQG
Structure of Tetracycline repressor in complex with antiinducer peptide-TAP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH MX-225 |
| Spacegroup name | I 41 |
| Unit cell lengths | 67.350, 67.350, 184.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.670 - 2.450 |
| R-factor | 0.18544 |
| Rwork | 0.183 |
| R-free | 0.23396 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ns8 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.972 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.600 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.050 | 0.650 |
| Number of reflections | 15051 | |
| <I/σ(I)> | 18.44 | 2.77 |
| Completeness [%] | 99.2 | 95.8 |
| Redundancy | 3.95 | 3.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | RESERVOIR: 3 M NACL, 0.1 M BIS-TRIS, PH 6.5 |
