3ZQF
Structure of Tetracycline repressor in complex with antiinducer peptide-TAP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH MX-225 |
Spacegroup name | I 41 |
Unit cell lengths | 68.650, 68.650, 182.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.180 - 2.560 |
R-factor | 0.208 |
Rwork | 0.206 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ns8 |
RMSD bond length | 0.024 |
RMSD bond angle | 2.366 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.710 |
High resolution limit [Å] | 2.560 | 2.560 |
Rmerge | 0.050 | 0.780 |
Number of reflections | 13467 | |
<I/σ(I)> | 17.78 | 1.53 |
Completeness [%] | 99.4 | 98.3 |
Redundancy | 4.21 | 4.16 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | RESERVOIR: 3 M NACL, 0.1 M BIS-TRIS, PH 6. |