3ZQB
PrgI-SipD from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 172.229, 48.055, 103.687 |
| Unit cell angles | 90.00, 121.94, 90.00 |
Refinement procedure
| Resolution | 32.890 - 2.400 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ym0 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.1.4) |
| Refinement software | CNS (1.21) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.540 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.080 | 0.500 |
| Number of reflections | 27970 | |
| <I/σ(I)> | 12.47 | 2.97 |
| Completeness [%] | 97.4 | 96.5 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | PROTEIN WAS MIXED WITH SOLUTION CONTAINING 0.49 M NA H2PO4 MONOHYDRATE AND 0.91 M K2 HPO4, PH 6.9. |
