3ZQ9
Structure of a Paenibacillus Polymyxa Xyloglucanase from Glycoside Hydrolase Family 44
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 83.610, 83.610, 157.531 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.230 - 1.860 |
| R-factor | 0.13837 |
| Rwork | 0.137 |
| R-free | 0.17122 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ykk |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.553 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.520 | 1.960 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.060 | 0.130 |
| Number of reflections | 54053 | |
| <I/σ(I)> | 26.3 | 11.3 |
| Completeness [%] | 99.5 | 96.6 |
| Redundancy | 7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25% (W/V) PEG 3350, 0.2M LITHIUM SULPHATE, 0.1M HEPES PH 6.5 |
