3ZQ4
Unusual, dual endo- and exo-nuclease activity in the degradosome explained by crystal structure analysis of RNase J1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 177.823, 209.430, 74.106 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.760 - 3.000 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bk1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.324 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.100 | 0.550 |
| Number of reflections | 55920 | |
| <I/σ(I)> | 11.4 | 2.2 |
| Completeness [%] | 98.2 | 98.9 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.2 M CACL2, 0.1 M TRIS.HCL, PH 8.0, 10 % (W/V) PEG 6000 |
