3ZPY
Crystal structure of the marine PL7 alginate lyase AlyA1 from Zobellia galactanivorans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-13 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 |
Unit cell lengths | 43.860, 50.390, 55.520 |
Unit cell angles | 69.14, 90.02, 84.92 |
Refinement procedure
Resolution | 43.660 - 1.430 |
R-factor | 0.16186 |
Rwork | 0.160 |
R-free | 0.20451 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uai |
RMSD bond length | 0.022 |
RMSD bond angle | 2.102 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.480 | 1.470 |
High resolution limit [Å] | 1.430 | 1.430 |
Rmerge | 0.080 | 0.220 |
Number of reflections | 78881 | |
<I/σ(I)> | 14.2 | 7.8 |
Completeness [%] | 96.1 | 94.2 |
Redundancy | 4.5 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 0.2 M KSCN AND 28% PEG-MME 2000, pH 7 |