3ZPY
Crystal structure of the marine PL7 alginate lyase AlyA1 from Zobellia galactanivorans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-13 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.860, 50.390, 55.520 |
| Unit cell angles | 69.14, 90.02, 84.92 |
Refinement procedure
| Resolution | 43.660 - 1.430 |
| R-factor | 0.16186 |
| Rwork | 0.160 |
| R-free | 0.20451 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uai |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.102 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.480 | 1.470 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.080 | 0.220 |
| Number of reflections | 78881 | |
| <I/σ(I)> | 14.2 | 7.8 |
| Completeness [%] | 96.1 | 94.2 |
| Redundancy | 4.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.2 M KSCN AND 28% PEG-MME 2000, pH 7 |
