3ZPN
Structure of Psb28
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-21 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 |
| Unit cell lengths | 57.060, 57.060, 183.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.060 - 2.361 |
| R-factor | 0.1945 |
| Rwork | 0.193 |
| R-free | 0.23420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2kvo |
| Data reduction software | XDS |
| Data scaling software | TRUNCATE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.060 | 2.420 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.050 | 0.760 |
| Number of reflections | 23633 | |
| <I/σ(I)> | 13.26 | 1.52 |
| Completeness [%] | 98.7 | 97.6 |
| Redundancy | 3.12 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 35% W/V PEG 3350, 300MM POTASSIUM THIOCYANATE |
