3ZPC

Acinetobacter baumannii RibD, form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-11-20
Detector	RIGAKU IMAGE PLATE
Spacegroup name	C 2 2 21
Unit cell lengths	148.987, 189.555, 76.406
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	117.140 - 2.200
R-factor	0.21469
Rwork	0.213
R-free	0.25037
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g6v
RMSD bond length	0.009
RMSD bond angle	1.474
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.340	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.090	0.440
Number of reflections	54441
<I/σ(I)>	6.4	1.9
Completeness [%]	99.0	99.7
Redundancy	2.9	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			PROTEIN BUFFER: 20 MM TRIS, 50 MM NACL, PH 7.5 RESERVOIR: 0.9M AMMONIUM PHOSPHATE, 0.075M SODIUM ACETATE HANGING DROPS