3ZOW
Crystal Structure of Wild Type Nitrosomonas europaea Cytochrome c552
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-24 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.450, 137.930, 204.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.220 - 2.350 |
| R-factor | 0.20406 |
| Rwork | 0.201 |
| R-free | 0.25765 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zox |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.911 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.800 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.060 | 0.300 |
| Number of reflections | 68939 | |
| <I/σ(I)> | 13.6 | 3.2 |
| Completeness [%] | 97.1 | 89.1 |
| Redundancy | 3.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 3 M AMMONIUM SULFATE, 10 MM TRIS-HCL PH 8 |
