3ZOP
Arg90Cit chorismate mutase of Bacillus subtilis at 1.6 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-03-23 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.134, 51.131, 69.906 |
| Unit cell angles | 97.84, 92.97, 101.26 |
Refinement procedure
| Resolution | 41.580 - 1.610 |
| R-factor | 0.17129 |
| Rwork | 0.169 |
| R-free | 0.22088 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dbf |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.203 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.470 | 1.710 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.020 | 0.100 |
| Number of reflections | 76808 | |
| <I/σ(I)> | 21.5 | 7.2 |
| Completeness [%] | 88.3 | 77 |
| Redundancy | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 100 MM MMT BUFFER ---MALIC ACID, MES, TRIS, IN MOLAR RATIOS OF 1:2:2, RESPECTIVELY--- PH 6.0, 150 MM CALCIUM CHLORIDE, 25% W/V PEG 1000 |
